| 质粒名称: | double_sgRNA targeting e1 and e4 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 190688 |
| 存储实验室: | Stanley Qi |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 空载体大小: | 5000 bp |
| 完整质粒大小: | 9290 bp |
| 载体类型: | 哺乳细胞、慢病毒，CRISPR，合成生物学表达 |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | sgRNAs targeting enhancer 1 and 4 of MYC separately |
| 物种来源: | H. sapiens (human) |
| GenBank编号 | NG_007161 |
| 标签/融合蛋白: |
BFP (C terminal on insert)
Puromycin (C terminal on insert
|
|
文献引用方法 |
|
| 材料和方法部分: |
double_sgRNA targeting e1 and e4 was a gift from Stanley Qi (Addgene plasmid # 190688 ; http://n2t.net/addgene:190688 ; RRID:Addgene_190688)
|
| 参考文献部分: | Nested epistasis enhancer networks for robust genome regulation. Lin X, Liu Y, Liu S, Zhu X, Wu L, Zhu Y, Zhao D, Xu X, Chemparathy A, Wang H, Cao Y, Nakamura M, Noordermeer JN, La Russa M, Wong WH, Zhao K, Qi LS. Science. 2022 Aug 11:eabk3512. doi: 10.1126/science.abk3512. 10.1126/science.abk3512 PubMed 35951677 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS double_sgRNA targeting e1 and e4 9279 bp ds-DNA circular SYN 19-OCT-2022
DEFINITION sgRNAs targeting enhancer 1 and 4 of MYC separately.
ACCESSION .
VERSION .
KEYWORDS double_sgRNA targeting e1 and e4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9279)
AUTHORS Lin X, Liu Y, Liu S, Zhu X, Wu L, Zhu Y, Zhao D, Xu X, Chemparathy
A, Wang H, Cao Y, Nakamura M, Noordermeer JN, La Russa M, Wong WH,
Zhao K, Qi LS
TITLE Nested epistasis enhancer networks for robust genome regulation.
JOURNAL Science. 2022 Aug 11:eabk3512. doi: 10.1126/science.abk3512.
PUBMED 35951677
REFERENCE 2 (bases 1 to 9279)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Oct 19, 2022 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9279
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1..17)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2..18)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 527..707
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(769..1357)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(849..868)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1528..2388)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2151..2170
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2389..2493)
/gene="bla"
/label=AmpR promoter
primer_bind complement(2568..2587)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 2759..3138
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 3140..3338
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 3293..3313
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 3356..3536
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 3583..3708
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 4205..4438
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 4623..4667
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 4965..5082
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 5335..5368
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 5372..5392
/label=mU6-F
/note="Mouse U6 promoter, forward primer"
primer_bind 5651..5671
/label=hU6-F
/note="Human U6 promoter, forward primer"
primer_bind 5822..5841
/label=LKO.1 5'
/note="Human U6 promoter, forward primer"
misc_RNA 5920..5995
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 6039..6250
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
intron 6251..7189
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 7146..7166
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 7210..7806
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(7210..7229)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 7706..7726
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
CDS 7816..7869
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 7882..8580
/codon_start=1
/product="monomeric blue fluorescent protein"
/label=TagBFP
/note="mammalian codon-optimized"
/translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
LPSKLGHKLN"
protein_bind 8600..8633
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 8689..9277
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8742..8762)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(9160..9171)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 cgataccgtc gacctcgatc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
61 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
121 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
241 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
301 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
361 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
421 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
481 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
541 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
601 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
661 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca tgtgagcaaa
721 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
781 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
841 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
901 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
961 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
1021 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
1081 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
1141 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
1201 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
1261 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
1321 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
1381 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
1441 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
1501 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
1561 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
1621 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
1681 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
1741 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
1801 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
1861 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
1921 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
1981 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
2041 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
2101 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
2161 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
2221 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
2281 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
2341 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
2401 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
2461 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
2521 cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac aatctgctct
2581 gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt cgctgagtag
2641 tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg catgaagaat
2701 ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata tacgcgttga
2761 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
2821 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
2881 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
2941 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
3001 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
3061 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
3121 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
3181 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
3241 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
3301 ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct gtactgggtc
3361 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
3421 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
3481 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
3541 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
3601 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
3661 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
3721 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
3781 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
3841 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
3901 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
3961 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
4021 aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag
4081 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
4141 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
4201 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
4261 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
4321 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
4381 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
4441 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
4501 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
4561 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
4621 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
4681 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
4741 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
4801 cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag
4861 aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac
4921 tgcgtgcgcc aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg
4981 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
5041 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
5101 agcagagatc cagtttggtt agtaccgggc ccgctctaga gatccgacgc gccatctcta
5161 ggcccgcgcc ggccccctcg cacggacttg tgggagaagc tcggctactc ccctgccccg
5221 gttaatttgc atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag
5281 ataatctgtt ctttttaata ctagctacat tttacatgat aggcttggat ttctataact
5341 tcgtatagca tacattatac gaagttataa acagcacaaa aggaaactca ccctaactgt
5401 aaagtaattg tgtgttttga gactataagt atcccttgga gaaccacctt gttggaacct
5461 tcagggaaaa ggcttgttta agagctaagc tggaaacagc atagcaagtt taaataaggc
5521 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct cgagtactag
5581 gatccattag gcggccgcgt ggataaccgt attaccgcca tgcagccttt tgctcattaa
5641 ttaaggtacc gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
5701 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
5761 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
5821 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
5881 gtggaaagcc agaaacatgg gctggctggg tctggtagtg ttttagagct agaaatagca
5941 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
6001 ttgaattcgc tagcctagag gtacctgtgc ccgtcagtgg gcagagcgca catcgcccac
6061 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
6121 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
6181 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
6241 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
6301 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
6361 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
6421 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
6481 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
6541 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
6601 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
6661 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
6721 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
6781 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
6841 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
6901 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
6961 gccgtccagg cacctcgatt agttctcgcg cttttggagt acgtcgtctt taggttgggg
7021 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
7081 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
7141 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc
7201 tagcccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
7261 cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
7321 gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
7381 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
7441 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
7501 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
7561 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
7621 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
7681 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
7741 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
7801 ggtgccggat cgggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
7861 cctggcccac cggtcgccac cagcgagctg attaaggaga acatgcacat gaagctgtac
7921 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
7981 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
8041 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag
8101 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
8161 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
8221 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
8281 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
8341 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc
8401 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
8461 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
8521 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaattaa
8581 gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt
8641 taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg
8701 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
8761 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
8821 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
8881 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
8941 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
9001 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
9061 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
9121 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
9181 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
9241 cagacgagtc ggatctccct ttgggccgcc tccccgcat
//
